ABSTRACT
A 3D printed (3DP) microfluidic polymerase chain reaction (PCR) device was demonstrated by detecting synthetic SARSCoV-2 at 106 copies/μL. The microfluidic device was fabricated using stereolithography 3DP and had a reaction volume of ~22 nL. The microdevice showed PCR amplification with 85 base synthetic ssDNA targets and primers designed for a SARS-CoV-2-specific region. The device was 2.5 times faster compared to a qPCR instrument with >60,000 times smaller reagent volume. The 3DP microdevice is a promising technology to significantly reduce the manufacturing costs of microfluidic devices that could be used towards point-of-care applications. © 2023 SPIE.
ABSTRACT
A 3D printed microarray device towards COVID 19 (SARS-COV-2) detection with a limit-of-detection of <167 nM was demonstrated. An array of 1,166 microwells, 116 x 116 μm in size, were 3D printed and synthetic targets and probes specific to COVID-19 spike-proteins were detected to demonstrate a device towards point-of-care COVID-19 detection. © COPYRIGHT SPIE. Downloading of the is permitted for personal use only.